Oligolabeling DNA probes to high specific activity with sequenase |
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| Authors: | Partha Sen Norimoto Murai |
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| Affiliation: | (1) Department of Plant Pathology and Crop Physiology, College of Agriculture, Louisiana State University, 70803-1720 Baton Rouge, LA, USA |
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| Abstract: | In this protocol we present a reproducible method of preparing DNA probes of high specific activity using Sequenase. The probes produced by this method had a specific activity of 2.8×109 cpm/μg with 69% of the total radioactivity incorporated into the TCA-precipitable materials. Probes with 5 to 10-fold lower specific activity were obtained using commercially available kits or using currently empolyed methods. |
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| Keywords: | Oligolabeling Sequenase |
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