|
|||||
|
|
Limulus ventral photoreceptors contain a homologue of the alpha-subunit of mammalian Ns |
|
| Authors: | C C Malbon U B Kaupp J E Brown |
| Institution: | 1. Department of Pharmacological Sciences and Department of Physiology and Biophysics, The State University of New York, Stony Brook, NY 11794-8651, USA, Schwerpunkt Biophysik, Universität Osnabrück, D-4500 Osnabrück, FRG and The Marine Biological Laboratory, Woods Hole, MA 02543, USA |
| Abstract: | Membranes from ventral photoreceptors of Limulus were incubated with cholera toxin and 32P]NAD+. Cholera toxin catalyzes a specific ADP-ribosylation of a 43-kDa peptide from Limulus ventral photoreceptors. Possible homologies between the 43-kDa peptide of Limulus and the alpha-subunits of mammalian stimulatory, guanine nucleotide-binding regulatory component of adenylate cyclase (Ns) were investigated by comparing the electrophoretic patterns of proteolytic fragments derived from each of these peptides that are radiolabeled by 32P]NAD+ and cholera toxin. Evidence is provided for structural homology between this invertebrate peptide and mammalian Ns. |
| Keywords: | Limulus Photoreceptor Cholera toxin ADP-ribosylation Stimulatory guanine nucleotide-binding regulatory component |
| 本文献已被 ScienceDirect 等数据库收录！ | |