大花金挖耳愈伤组织诱导与增殖

以大花金挖耳无菌苗的子叶、下胚轴和根为外植体,进行愈伤组织诱导与增殖研究。结果表明:大花金挖耳无菌苗的根是诱导愈伤组织的理想外植体;其愈伤组织诱导的最适培养基为:B5 3.0mg/L NAA 0.2mg/L6-BA,诱导率可达100%;愈伤组织的增殖在45g/L的蔗糖、pH5.7、光照12h/d培养条件下可延迟愈伤组织褐化出现的时间,并维持其良好的组织结构,愈伤组织的最适继代周期为30~40d。

Induction and Proliferation of Carpesium macrocephalum

The cotyledons,hypocotyls and roots of \?rpesium macrocephalum\% Franch.et Sav. seedlings were used as the explants to study callus induction and proliferation of \%C.macrocephalum\%.The results indicated that the roots of aseptic \%C.macrocephalum\% seedlings were the ideal explants of callus induction.Their optimal medium for inducing the roots to form calluses was B\-5 3.0 mg/L NAA 0.2 mg/L 6-BA and its callus induction rate reached as high as 100%.The calluses were cultured with sucrose at 45 g/L,pH at 5.7 and light at 12 hours per day to proliferate and this could delay their browning time and keep their tissue structures fine.In the meantime,the optimal period of the secondary culture of the calluses was 30~40 days.