In vitro multiplication ofVanilla planifolia using axillary bud explants |
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Authors: | P S George G A Ravishankar |
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Institution: | (1) Plant Cell Biotechnology Department, Central Food Technological Research Institute, 570013 Mysore, India;(2) Present address: Department of Botany, St. Joseph's College, Devagiri, Calicut, 673008 Kerala, India |
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Abstract: | A clonal propagation method has been developed for efficient multiplication ofVanilla planifolia. Multiple shoots were developed from axillary bud explants using semi-solid Murashige and Skoog (MS) medium supplemented with N6-benzyladenine (BA, 2 mg l–1) and -naphthaleneacetic acid (NAA, 1 mg l–1). The multiple shoots were transferred to agitated liquid MS medium with BA at 1 mg l–1 and NAA at 0.5 mg l–1 for 2–3 weeks, and subsequently cultured on semi-solid medium. Using this method, an average of 42 shoots were obtained from a single axillary bud explant over a period of 134 days. Use of an intervening liquid medium has been found to enhance multiplication of shoots inV. planifolia.Abbreviations
BA
N6-benzyladenine
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DMRT
Duncan's multiple-range test
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KC
Knudson (1946) medium
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KCB
KC basal medium
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Kn
kinetin
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MS
Murashige and Skoog (1962) medium
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MSB
MS basal medium
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1/2 MSB
half-strength MSB
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MS-D
double-phase MS medium
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MS-L
liquid MS medium
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MS-S
semi-solid MS medium
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NAA
-Naphthaleneacetic acid |
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Keywords: | Vanilla planifolia Axillary bud Liquid media Multiple shoots Plantlets |
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