High resolution melting analysis for detection of variable number of tandem repeats polymorphism of XRCC5 |
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| Authors: | Mehrdad Rajaei Iraj Saadat Mostafa Saadat |
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| Institution: | Department of Biology, College of Sciences, and Institute of Biotechnology, Shiraz University, Shiraz, Iran. |
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| Abstract: | Several polymorphisms in the XRCC5 (X-ray repair cross-complementing 5; OMIM: 194364) were reported. Polymorphism of variable number of tandem repeats (VNTR) in the promoter region of XRCC5 (rs6147172) was reported. The main aim of the present study is to introduce the high resolution melting analysis (HRMA) method for genotyping of the polymorphism of XRCC5 VNTR. Genotypes of XRCC5 VNTR were determined by HRMA and conventional PCR method, and confirmed by DNA sequencing. The results for genotyping using HRMA and conventional PCR showed 100% concordance. All genotypes of the XRCC5 VNTR polymorphism could be accurately detected by HRMA. |
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