Ginseng root water-extracted pectic polysaccharides originate from secretory cavities |
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Authors: | Li Yu Yifa Zhou J Paul Knox |
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Institution: | (1) School of Life Sciences, Northeast Normal University, Changchun, 130024, People’s Republic of China;(2) Faculty of Biological Sciences, Centre for Plant Sciences, University of Leeds, Leeds, LS2 9JT, UK; |
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Abstract: | A range of molecular probes for cell wall polysaccharides has been used to explore the structure and location of water-extracted
pectic polysaccharides occurring in fractions isolated from ginseng roots. The LM19 homogalacturonan (HG) epitope was abundant
in an HG fraction and analysis of LM19 binding to a rhamnogalacturonan-I (RG-I) rich-fraction indicated that the LM19 epitope
is sensitive to acetylation. A specific RG-I epitope (LM16), four arabinogalactan-protein (AGP) epitopes (LM2, LM14, JIM16,
MAC207) and an extensin epitope (JIM20) were found to be abundant and co-located in several isolated polysaccharide fractions
including an arabinogalactan fraction and two RG-I fractions. Detection of the RG-I, AGP and extensin epitopes identified
in isolated polysaccharide fractions in sections of ginseng roots indicated that they were most abundant in secretory cavities
found in the cortical regions of ginseng roots. In addition, the immunocytochemical study indicated that polysaccharide epitope
masking is a widespread phenomenon in the primary cell walls of ginseng roots. |
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