| 纤维素酶中具有壳聚糖水解酶活性成分的鉴定 |
| |
| 引用本文： | 刘靖,夏文水.纤维素酶中具有壳聚糖水解酶活性成分的鉴定[J].中国生物化学与分子生物学报,2005,21(5):712-715. |
| |
| 作者姓名： | 刘靖 夏文水 |
| |
| 作者单位： | 1. 江南大学食品科学与安全教育部重点实验室,江苏,无锡,214036;江苏畜牧兽医职业技术学院,江苏,泰州,225300
2. 江南大学食品科学与安全教育部重点实验室,江苏,无锡,214036 |
| |
| 基金项目： | 国家自然科学基金资助项目N0.20271023~~ |
| |
| 摘 要： | 在壳聚糖酶的研究过程中,目前已发现37种酶具有非专一性地降解壳聚糖的能力1].对这些非专一性酶水解壳聚糖的机理有两种看法:一些人认为,由于这些酶大都来自商业酶制剂,未经过进一步的纯化,故有人认为其中所含的少量杂质可能是产生水解活力的原因;但也有人认为,在所有的酶制剂中都存在同一种杂质似乎是不可能的,因为这些酶来源于广泛的微生物、真菌、哺乳动物和植物等.众所周知,酶具有高度的专一性,即对所催化的反应和底物有严格的选择性,一种酶往往只能催化一种或一类反应;有如此多的不同种类的酶能非专一性地水解壳聚糖.因而探讨具有水解…
|
| 收稿时间： | 2005-10-20 |
| 修稿时间： | 2005年1月24日 |
Characterization of Chitosanase from Cellulase Produced by Trichoderma viride |
| |
| LIU Jing,XIA Wen-Shui.Characterization of Chitosanase from Cellulase Produced by Trichoderma viride[J].Chinese Journal of Biochemistry and Molecular Biology,2005,21(5):712-715. |
| |
| Authors: | LIU Jing XIA Wen-Shui |
| |
| Institution: | (1) Key Laboratory of Food Science and safety, Ministry of Education, Southern Yangtze University, Wuxi 214036, China; 2) Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China |
| |
| Abstract: | Chitosanase which catalyze the hydrolytic degradation of chitosan is regarded as an important enzyme for the extense carbon and nitrogen recycle which occurs in nature. A bifunctional enzyme with chitosanase and carboxymethyl cellulase (CMCase) activity was purified from commercial cellulose, which was produced by Trichoderma viride, through sequential steps of DEAE-Sepharose CL-6B, ion-exchange chromatography, phenyl Sepharose CL-4B hydrophobic chromatography and Sephacryl S-200 gel filtration. The purified hydrolase was homogeneous, as examined by SDS-PAGE and RP-HPLC. The molecular mass was 66 kD and 64 kD estimated by SDS-PAGE and gel filtration,respectively. |
| |
| Keywords: | carboxymethyl cellulase chtiosanase bifunctional activity characterization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
|
