| 重组人超氧化物歧化酶化学修饰的初步研究 |
| |
| 引用本文： | 骆训懿,王晶翼,谢邦铁,李战青,刘晓琳,陈晓穗.重组人超氧化物歧化酶化学修饰的初步研究[J].生物化学与生物物理进展,1993,20(6):452-456. |
| |
| 作者姓名： | 骆训懿 王晶翼 谢邦铁 李战青 刘晓琳 陈晓穗 |
| |
| 作者单位： | 海军总医院分子生物学研究室;海军总医院分子生物学研究室;海军总医院分子生物学研究室;海军总医院分子生物学研究室;海军总医院分子生物学研究室;海军总医院分子生物学研究室 |
| |
| 摘 要： | 在高效表达重组人铜锌超氧化物歧化酶(rh Cu/Zn SOD),并纯化得到比活大于4000单位的 rh Cu/Zn SOD 纯品的基础上,采用活化酯法将聚乙二醇(PEG)与 rhCu/Zn SOD 交联,获得分子量约6万的 PEG-SOD 交联物.经 PEG 修饰的酶稳定性增强,表现为对酸、碱和热的耐受力均较未交联酶高.修饰酶的生物半衰期为15h,是天然酶的90倍,酶活性保留80%以上.还实验观察了修饰剂用量与修饰酶保留活性之间的关系.
|
| 关 键 词： | 超氧化物歧化酶 蛋白质化学修饰 聚乙二醇 基因工程 |
| 收稿时间： | 1992/9/22 0:00:00 |
| 修稿时间： | 1993/2/12 0:00:00 |
A Preliminary Study on Chemical Modification of Recombinent Human Superoxide Dismutase |
| |
| Luo Xunyi,Wang Jingyi,Xie Bangtie,Li Zanqing,Liu Xiaoling and Chen Xiaosui.A Preliminary Study on Chemical Modification of Recombinent Human Superoxide Dismutase[J].Progress In Biochemistry and Biophysics,1993,20(6):452-456. |
| |
| Authors: | Luo Xunyi Wang Jingyi Xie Bangtie Li Zanqing Liu Xiaoling and Chen Xiaosui |
| |
| Institution: | Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital |
| |
| Abstract: | To overcome several disadvantages of the use of superoxide dismutase(SOD)in its native form the surface modification of therapeutically useful enzyme by covalent linkage of nontoxic,nonimmunogenic, and biocompatible polymers has been investigated.Recombinent human Cu/Zn SOD (rh Cu/Zn SOD) obtained from E.coli was coupled chemically with activated polyethylene glycol(PEG).The modified rhCu/Zn SOD,with apparent molecular weight about 60000 daltons,has longer half-life(15 hours), and the remaining enzyme activity is more than 80 per cent. Its stabilities against acid, alkali and heat were increased significantly. The relation between the amount of PEG used and the remained enzyme activity was also reported. |
| |
| Keywords: | superoxide dismutase chemical modification PEG genetic engineering |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
