Molecular detection of Candidatus Rickettsia asembonensis in fleas collected from pets and domestic animals in Puducherry,India |
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| Authors: | N Nataraj K Muthuraman D Sundaram E Ayyanar M Ashokkumar G Kasinathan P Devaraju |
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| Institution: | 1. Division of Vector Biology and Control, ICMR-Vector Control Research Centre (ICMR-VCRC), Indira Nagar, Puducherry, India;2. Division of Microbiology & Molecular Biology, ICMR-Vector Control Research Centre (ICMR-VCRC), Indira Nagar, Puducherry, India |
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| Abstract: | Rickettsia are obligate intracellular pathogens transmitted by arthropod vectors. The re-emergence of several rickettsioses imposes severe global health burden. In addition to the well-established rickettsial pathogens, newer rickettsial species and their pathogenic potentials are being uncovered. There are many reports of spotted and typhus fever caused by rickettsiae in India. Hence, in this study we screened the ectoparasites of pet and domestic animals for the presence of rickettsia using polymerase chain reaction. Nine cat flea samples (Ctenocephalides felis felis), that tested positive for the presence of rickettsia were subjected to Multi Locus Sequence Typing. Nucleotide sequencing and Phylogenetic analysis of gltA, ompB and 16rrs genes revealed that the rickettsiae detected in cat fleas was Rickettsia asembonensis. Further studies are required to assess Rickettsia asembonensis pathogenic potential to human and its enzootic maintenance of in various hosts and vectors. |
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| Keywords: | Rickettsia asembonensis 16rrs cat flea gltA ompB Puducherry RFLO |
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