| CpG联合铝佐剂对HCV免疫原体液免疫作用的影响 |
| |
| 引用本文： | 湛娜,修冰水,王国华,陈堃,白冠中,宋晓国,朱翠霞,戴振华,杨锡琴,张贺秋.CpG联合铝佐剂对HCV免疫原体液免疫作用的影响[J].生物技术通讯,2011,22(6):777-780. |
| |
| 作者姓名： | 湛娜 修冰水 王国华 陈堃 白冠中 宋晓国 朱翠霞 戴振华 杨锡琴 张贺秋 |
| |
| 作者单位： | 1. 军事医学科学院基础医学研究所,附属医院,北京100071;军事医学科学院附属医院,北京100071
2. 军事医学科学院基础医学研究所,附属医院,北京100071 |
| |
| 基金项目： | "十一五"国家科技重大专项 |
| |
| 摘 要： | 目的:探讨胞苷酸鸟苷寡脱氧核苷酸(CpG ODN)联合铝佐剂对丙型肝炎病毒(HCV)重组免疫原的体液免疫作用。方法:采用高交叉HCV-HVR1和E1重组蛋白与CpG ODN、铝佐剂组合,免疫BALB/c小鼠后以ELISA、酶联免疫斑点测定、流式细胞术、免疫沉淀等方法检测相关体液免疫指标和免疫血清多抗的交叉反应性。结果:CpG联合铝佐剂激发了最高的特异性抗体滴度;佐剂通过提高抗体分泌细胞数量、增加脾脏中记忆B细胞数量、增加脾淋巴细胞IL-6、IL-10分泌浓度实现体液免疫增效;CpG则能提高免疫效率,联合铝佐剂时显著提高浆细胞数量;12份HCV阳性血清中有10份可与多抗HVR1 IgG发生免疫沉淀。结论:CpG和铝佐剂联合应用具有协同作用,多抗HVR1 IgG具有较好的交叉反应性。
|
| 关 键 词： | 丙型肝炎病毒 体液免疫 免疫佐剂 胞苷酸鸟苷寡脱氧核苷酸 |
Enhancement of Humoral Immunity in the Mouse by Coupling CpG and Aluminium to the HCV Recombinant Immunogen |
| |
| ZHAN Na,XIU Bing-Shui,WA NG Guo-Hua,CHEN Kun,BAI Guan-Zhong,SONG Xiao-Guo,ZHU Cui-Xia,DAI Zhen-Hua,YANG Xi-Qin,ZHANG He-Qiu.Enhancement of Humoral Immunity in the Mouse by Coupling CpG and Aluminium to the HCV Recombinant Immunogen[J].Letters in Biotechnology,2011,22(6):777-780. |
| |
| Authors: | ZHAN Na XIU Bing-Shui WA NG Guo-Hua CHEN Kun BAI Guan-Zhong SONG Xiao-Guo ZHU Cui-Xia DAI Zhen-Hua YANG Xi-Qin ZHANG He-Qiu |
| |
| Institution: | ZHAN Na1,2,XIU Bing-Shui1,WANG Guo-Hua1,CHEN Kun1,BAI Guan-Zhong1,SONG Xiao-Guo1,ZHU Cui-Xia1,DAI Zhen-Hua1,YANG Xi-Qin1,ZHANG He-Qiu1 1.Institute of Basic medical Sciences,Academy of Military Medical Sciences,Beijing 100850,2.Hospital 307 of PLA,Beijing 100071,China |
| |
| Abstract: | Objective: To investigate the enhancement of humoral immunity when cytidine phosphate guanosine oligodeoxynucleotides(CpG ODN) and aluminium adjuvants were complexed with the HCV recombinant immunogen in mice.Methods: After immunizing BALB/c mice with the recombination HCV antigen adjuvanted with pUCpGs10 and/or aluminium(antigen+CpG+alum,antigen+CpG,antigen+alum,antigen+PBS),ELISA,enzyme-linked immunospot assay and flow cytometry were used to measure the indexes of humoral immunity.Results: The aluminium a... |
| |
| Keywords: | hepatitis C virus humoral immunity adjuvant cytidine phosphate guanosine oligodeoxynucleotide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
