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| 开放条件下高聚β-羟基丁酸酯含量的甲烷氧化混合菌群的富集 | |
| 引用本文： | 姜丹,董静,辛嘉英,张莹莹,张颖鑫,夏春谷.开放条件下高聚β-羟基丁酸酯含量的甲烷氧化混合菌群的富集[J].生物技术通讯,2011,22(6):846-849. |
| 作者姓名： | 姜丹 董静 辛嘉英 张莹莹 张颖鑫 夏春谷 |
| 作者单位： | 1. 哈尔滨商业大学食品科学与工程省重点实验室,黑龙江哈尔滨150076; 2. 哈尔滨商业大学食品科学与工程省重点实验室,黑龙江哈尔滨150076;中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000 3. 哈尔滨商业大学食品科学与工程省重点实验室,黑龙江哈尔滨,150076 4. 中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州,730000 |
| 基金项目： | 国家自然科学基金，黑龙江省教育厅重点项目 |
| 摘 要： | 目的:在开放条件下以甲烷为底物富集高聚β-羟基丁酸酯(PHB)含量的甲烷氧化菌群,获得能够利用低成本碳源高产PHB的菌种。方法:采用丰盛-饥饿模式间歇供料,以甲烷为底物,好氧开放式培养甲烷氧化混合菌群,利用苏丹黑染色法动态检测丰盛和饥饿阶段胞内PHB含量的变化,以此为基础考察丰盛-饥饿期比例对富集高PHB含量的甲烷氧化菌群的影响。结果:丰盛-饥饿期比例为1∶3时,微生物PHB含量从17.7%增加到35.5%,且开放培养过程中菌群结构稳定。结论:通过丰盛-饥饿模式间歇供料开放式培养所得的高PHB含量的稳定的甲烷氧化菌群,具有工业生产PHB的应用价值。 |
| 关 键 词： | 聚β-羟基丁酸酯 苏丹黑 富集 甲烷氧化混合菌HD6T |
Enrichment of Methane-Utilizing Mixed Culture with a High Polyβ-Hydroxybutyrat Content in an Open System |
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| JIANG Dan,DONG Jing,XIN Jia-Ying,ZHANG Ying-Ying,ZHANG Ying-Xin,XIA Chun-Gu.Enrichment of Methane-Utilizing Mixed Culture with a High Polyβ-Hydroxybutyrat Content in an Open System[J].Letters in Biotechnology,2011,22(6):846-849. | |
| Authors: | JIANG Dan DONG Jing XIN Jia-Ying ZHANG Ying-Ying ZHANG Ying-Xin XIA Chun-Gu |
| Institution: | JIANG Dan1,DONG Jing1,XIN Jia-Ying1,2,ZHANG Ying-Ying1,ZHANG Ying-Xin1,XIA Chun-Gu2 1.Key Laboratory for Food Science and Engineering,Harbin University of Commerce,Harbin 150076,2.State Key Laboratory for Oxo Synthesis & Selective Oxidation,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China |
| Abstract: | Objective： To enrich a methane-utilizing mixed culture with a high poly-β-hydroxybutyrate（PHB） content using methane as substrate in an open system,then obtain microbial species of high PHB content at low carbon cost.Methods： The method of feast-famine aerobic dynamic feeding was used to cultivate methane-utilizing mixed culture using methane as substrate in an open system,and the effect of feast-famine ratio on enriching mixed culture with a high PHB content was studied based on sudan black staining.Results： The PHB content in biomass increased from 17.7% to 35.5% when the feast-famine ratio was 1∶3,and the structure of mixed culture was stable in an open system.Conclusion： Methane-Utilizing mixed culture enriched by the method of feast-famine aerobic dynamic feeding has the application value for commercial PHB production. |
| Keywords: | poly-β-hydroxybutyrate sudan black enrichment Methylococcus capsulatus HD6T |
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