艰难梭菌毒素A对胆管癌细胞株FRH-0201增殖和凋亡的影响

目的：研究艰难梭菌毒素A（TcdA）对人胆管癌细胞株FRH-0201细胞凋亡影响及作用机制。方法：采用MTT法检测细胞增殖抑制率,荧光染色检测TcdA作用后细胞形态学变化,流式细胞术检测细胞凋亡,免疫细胞化学法检测Bcl-2表达水平,Caspase3活性检测试剂盒检测Caspase-3的活性。结果：TcdA能抑制胆管癌细胞株FRH-0201细胞增殖且呈时间、剂量依赖性,荧光染色和流式细胞仪检测到细胞凋亡,与对照组相比,TcdA作用后Bcl-2蛋白表达下降,差异有统计学意义（P〈0.05）,Caspase-3活性增强,差异有统计学意义（P〈0.05）。结论：TcdA可以通过下调Bcl-2蛋白表达表达,激活Caspase-3而诱导FRH-0201细胞凋亡。

Effect of Clostridium DifficileToxin A on Proliferation and Apoptosis of FRH-0201 Cholangiocarcinoma Cell Line

Objective： To investigate the proliferation inhibition and apoptosis effect of Clostridium difficile toxin A on the cholangiocarcinoma cell lines FRH-0201.Methods： The cytotoxic activity of TcdA on FRH-0201 cells was tested by the MTT assay,Morphological changes were observed by fluorescence microscopy.Apoptosis was examined by flow-cytometric analysis.The expres-sion of Bcl-2 protein was examined by SABC immunocytochemistry staining,and Caspase-3 activity was determined with a microplate reader.Results： TcdA inhibited the proliferation of FRH-0201 cell line in a time-and dose-dependent manner,the cells presented the typ-ical morphological changes of apoptosis,the expression of Bcl-2 protein was down-regulated compared with control group（P0.05）,in addition,Caspase-3 was activated significantly compared with control group（P0.05）.Conclusions： TcdA can induce apoptosis in FRH-0201 cells by the down-regulation of Bcl-2 protein and activity of Caspase-3.