Protein carbonylation in barley seedling roots caused by aluminum and proton toxicity is suppressed by salicylic acid |
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Authors: | H Song X Xu H Wang Y Tao |
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Institution: | 1.The Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou,China;2.College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou,China |
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Abstract: | Proton (H+) and aluminum (Al3+) toxicities are major factors limiting crop production on acid soils. To study whether salicylic acid (SA) is functional
in alleviating protein damage caused by H+ and Al3+ toxicities, an investigation of the antioxidant defense response regulated by SA was carried out on barley (Hordeum vulgare L.) seedlings under H+, Al3+, and combined stresses. It was found that the relative root elongation of seedlings, which grew in the solutions supplemented
with SA, was significantly higher than that of seedlings without SA treatment after 24-h treatments with H+, Al3+, and combined stresses. The lesser amount of carbonylated proteins with molecular weights ranging from 14.4 to 97 kD, was
accumulated in seedlings treated with SA than that in the seedlings without SA treatment. The higher activities of antioxidant
enzymes and lesser content of MDA were observed in seedlings treated with SA compared with the seedlings without SA treatment.
Moreover, the nitroblue tetrazolium staining of roots showed that ROS accumulation was decreased by SA treatments. This study
suggested that SA could alleviate cell damage caused by H+ and Al3+ toxicities on acid soils by both activating antioxidant defense responses and reducing the contents of carbonylated proteins
caused by ROS in barley seedlings. |
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