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Mapping the order of DNA restriction fragments |
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| Authors: | W M Fitch T F Smith W W Ralph |
| Affiliation: | 1. Central Institute of Molecular Biology, Department of Virology, Academy of Sciences of the GDR, DDR-1115 Berlin-Buch, Robert-Rössle-Strasse 10 G.D.R. Tel. 3462328;2. D.I. Ivanovsky Institute of Virology, Academy of Medical Sciences of the USSR, Department of Biochemistry, Moscow 113098 U.S.S.R. Tel. 1902851;3. Department of Molecular Biophysics, Academy of Sciences of the GDR, DDR-1115 Berlin-Buch, Robert-Rössle-Strasse 10 G.D.R. Tel. 3462328 |
| Abstract: | A straightforward method was designed for mapping the order of DNA restriction fragments obtained by a double and two single digestions, without the necessity of using a computer or a radioactive label. All possible solutions compatible with a pre-set level of error in the determination of sequence lengths are obtained. The primary assumptions are given, and the appropriate modifications of the algorithm are presented as a function of any assumptions one is unable (or unwilling) to make. Use of the method in connection with end-labeled fragments is also described. |
| Keywords: | Recombinant DNA brain tumors restriction endonuclease mapping Southern hybridization electron microscopy heteroduplex analysis bp base pairs CPE cytopathic effect GBM1, GBM3 DNA SV40-GBM DNA isolated after one or three passages of the virus in cell culture H- and L-form heavy and light forms of GBM3 DNA kb kilobases or kilobase pairs SV40-GBM SV40-like virus isolated from a human glioblastoma multiforme |
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