Isolation and characterization of a novel nuclear protein from pollen mother cells of lily |
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Authors: | Sasaki Y Yasuda H Ohba Y Harada H |
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Affiliation: | Institute of Biological Sciences, University of Tsukuba, Ibaraki 305, Japan. |
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Abstract: | Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G2 period of premeiosis and is present in mature pollen. PMCP and H1 were extracted from pollen mother cells with 5% perchloric acid and isolated by reverse-phase high-performance liquid chromatography. The amino acid composition of PMCP differs from that of somatic H1. However, PMCP is similar to H1t in mammalian testis with regard to amino acid composition. |
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