南荻的组织培养与快速繁殖技术

以南荻幼穗为外植体,进行了组织培养与快速繁殖技术的研究。结果表明：最佳诱导培养基：MS＋2.0mg·L-12,4-D＋0.1mg·L-16-BA＋0.5g·L-1水解酪蛋白＋0.5g·L-1脯氨酸;最佳分化培养基：MS＋0.5mg·L-1NAA＋0.5mg·L-1KT＋0.5mg·L-1IAA＋2.0mg·L-16-BA＋0.5g·L-1水解酪蛋白＋0.5g·L-1脯氨酸;最佳生根培养基：MS＋1.0mg·L-1NAA＋0.25mg·L-1Met。炼苗后,移入营养土与珍珠岩（1：1）的基质中,移栽成活率高达100%。该体系的建立为南荻规模化生产及遗传改良提供了前提条件。

Tissue Culture and Rapid Propagation Technology of Triarrhena lutarioriparia L.Liu sp.nov.

Young ears of Triarrhena lutarioriparia were used as explant in the study of tissue culture and rapid propagation.The results indicate that the best induced medium for callus induction was MS medium with 2.0 mg·L-1 2,4-D,1.0 mg·L-1 6-BA,0.5 g·L-1 casein acids hydrolysate and 0.5 g·L-1 proline.The best medium for bud differentiation was MS medium with 0.5 mg·L-1 NAA,0.5 mg·L-1 KT,0.5 mg·L-1 IAA,2.0 mg·L-1 6-BA,0.5 g·L-1 casein acids hydrolysate and 0.5 g·L-1 proline.The best medium for root differentiation was MS medium with 1.0 mg·L-1 NAA and 0.25 mg·L-1Met.After acclimatization,the culture-bottle seedlings were transplanted into base materials which contains nutritious soil and perlite（1：1）.The survival rate was up to 100%.The established system provided preconditions for large-scale production and genetic modification improvement of Triarrhena lutarioriparia.