Reduction of methemoglobin with photoradicals of benzophenone-3,3',4,4'-tetracarbonic acid

Reaction ability of partially reduced human methemoglobin was estimated by the rate of protein addition of carbon monoxide immediately after reduction. Impulse of photoexcitation allowed two variants of these experiments to be carried out: immediately after partial reduction of methemoglobin with photoradicals of benzophenone 3,3',4,4'-tetracarbon acid and after photodissociation of the carboxy-complex obtained in the first case. It has been shown that at protein reduction "p" less than or equal to 0.03 under these conditions the initially obtained R-form of protein is partially transformed into the less reactive T-form.