Mureinolytic ability of the rumen ciliate <Emphasis Type="Italic">Diploplastron affine</Emphasis> |
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| Authors: | G Be??ecki R Miltko E Kwiatkowska T Micha?owski |
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| Institution: | (1) Center for Molecular and Biomolecular Informatics, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands;(2) Institute of Rural Sciences, University of Wales, SY23 3AL Aberystwyth, UK;(3) I.N.R.A., Station de Recherches sur la Nutrition des Herbivores, Centre de Recherches de Clermont, Ferrand/Theix, France;(4) National Institute of Livestock and Grassland Science, 2 Ikenodai, Kukizaki, 305-0901 Ibaraki, Japan;(5) Rowett Research Institute, AB21 9SB Aberdeen, UK;(6) Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jablonna, Warsaw, Poland;(7) Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba, 305-0854 Tsukuba, Japan;(8) Laboratory of Animal Science, Kyoto Prefectural University, Shimogamo, 606-8522 Kyoto, Japan;(9) Department of Evolutionary Microbiology, Radboud University Nijmegen, Nijmegen, The Netherlands |
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| Abstract: | Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide
of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated from the rumen fluid and grown in vitro or inoculated into the rumen of defaunated sheep. The results of long-term cultivation of protozoa showed a positive correlation
between their number and murein content in the culture medium. It was also found that bacteria-free D. affine ciliates incubated with or without murein produced volatile fatty acids at the rate of 12.3 and 8.7 pmol/h per protozoan,
respectively, acetic, butyric and propionic acids being the three main acids released to the medium. Enzyme studies performed
with the use of protozoan cell extract prepared from bacteria-free ciliates degraded murein at a rate of 25 U/mg protein per
h; two mureinolytic enzymes were identified by zymographic technique in the examined preparation. |
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