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| 水霉菌的形态及ITS区分子鉴定 | |
| 引用本文： | 可小丽,汪建国,顾泽茂,李明,龚小宁.水霉菌的形态及ITS区分子鉴定[J].水生生物学报,2010,34(2):293-301. |
| 作者姓名： | 可小丽 汪建国 顾泽茂 李明 龚小宁 |
| 作者单位： | 1. 中国科学院水生生物研究所,武汉,430072;中国科学院研究生院,北京,100049 2. 中国科学院水生生物研究所,武汉,430072 3. 华中农业大学水产学院,武汉,430070 4. 武汉工业学院饲料科学系,武汉,430023 |
| 基金项目： | 湖北省科技攻关计划项目，973计划项目，中国科学院领域前沿项目 |
| 摘 要： | 研究利用形态学和分子生物学相结合的方法, 对从患病黄颡鱼病灶处分离的水霉菌株(HSY)的分类地位进行了研究。结果显示, 该菌在18—23℃均能形成游动孢子囊, 但只在20℃才大量释放游动孢子; 在15℃培养约3 周出现大量的藏卵器, 多数顶生, 形态与Saprolegnia litoralis 和Saprolegnia ferax 的均非常相似。序列比对分析显示, HSY 菌株与S. litoralis、S. bulbosa、S. oliviae、S. longicaulis、S. ferax、S. mixta 和S.anomalies 的ITS 区(包括5.8S rDNA)序列相似性均高于99%。系统发育分析显示, 所选的真菌分为3 个群,HSY 落于第一个群, MP 树显示HSY 菌株与S. litoralis、S. bulbosa、S. oliviae、S. longicaulis、S. ferax 以及S. mixta 枝系均互相平行, 而NJ 树显示其与S. ferax 和S. mixta 更近。综合形态学、ITS 区序列及系统发育分析, 将HSY 菌株暂定为多子水霉(Saprolegnia ferax)。
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| 关 键 词： | 卵菌纲 水霉 分类 ITSrDNA 系统发育 |
SAPROLEGNIA IDENTIFICATION BASED ON THEIR MORPHOLOGICAL CHARACTERISTICS AND ITS rDNA REGION |
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| KE Xiao-Li,WANG Jian-Guo,GU Ze-Mao,LI Ming,GONG Xiao-Ning.SAPROLEGNIA IDENTIFICATION BASED ON THEIR MORPHOLOGICAL CHARACTERISTICS AND ITS rDNA REGION[J].Acta Hydrobiologica Sinica,2010,34(2):293-301. | |
| Authors: | KE Xiao-Li WANG Jian-Guo GU Ze-Mao LI Ming GONG Xiao-Ning |
| Institution: | KE Xiao-Li1,2,WANG Jian-Guo1,GU Ze-Mao3,LI Ming4 , GONG Xiao-Ning1 (1. Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,2. The Graduate University of the Chinese Academy of Sciences,Beijing 100049,3. College of Fisheries,Huazhong Agricultural University,Wuhan 430070,4. Feed Science Department,Wuhan Polytechnic University,Wuhan 430023) |
| Abstract: | Saprolegnia is one of the main genus of water molds responsible for fungal infections of freshwater fish and their eggs. These infections are usually termed saprolegniasis, which can cause severe losses of freshwater fish in both nature and commercial fish farms. However, identification of the causative agent is often unmanageable, especially to those isolated from fish lesions. Traditionally, the generic definition was mainly based on the asexual characters, espe-cially the mode of zoospores discharge, and... |
| Keywords: | ITS rDNA Oomycetes Saprolegnia Taxonomy ITS rDNA Phylogenetics |
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