三七皂苷对缺氧复氧心肌细胞损伤保护作用的研究

目的：研究三七皂苷对纯化培养大鼠乳鼠心肌细胞缺氧/复氧损伤的保护作用及机制。方法：采用纯化培养的心肌细胞建立缺氧/复氧损伤模型,测定细胞凋亡率、caspase-3、乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）含量。结果：与正常组比较,模型组LDH、MDA含量、caspase-3活性及细胞凋亡率明显升高（P＆lt;0.01）,SOD活性明显降低（P＆lt;0.01）;三七皂苷组降低LDH、MDA含量、caspase-3活性和细胞凋亡率,提高SOD活性,与缺氧/复氧组比较各实验指标差异均具有显著性（P＆lt;0.05）。结论：三七皂苷对缺氧/复氧心肌细胞损伤有保护作用,作用机制与清除氧自由基,抗脂质过氧化及降低细胞凋亡率有关。

Protective effects of Panax notoginseng saponins on neonatal rat cardiomyocytes exposed to hypoxia-reoxygenation injury

Objective： To investigate the cardioprotective effects of Panax notoginseng saponins on pure cultrared neonatal rat cardiomyoeytes exposed to hypoxia-reoxygenation （H/R） injury and the mechanisms. Methods： The hypoxia/reoxygenation injury model of pure cultured neonatal rat eardiomyocytes was employed. The concentration of lactate dehydrogenase （LDH） and malonialdehvde （MDA）, the activity of super-oxide dismutes（SOD） and caspase-3, the apoptosis rate of cardiomyocyte were detected. Results： Compared with the control group, the model group showed lower activety of SOD and higher concentrations of LDH, MDA, the apoptosis rate and activity ofcaspase-3 （p〈0.01）. After treated with Panax notoginseng saponins （PNS）, the activity of SOD was significantly increased but the concentration of LDH and MDA, the activety ofcaspase-3 and the apoptosis rate were decreased （p〈0.05）. Conclusions： PNS could protect cardiomyocytes against H/R injury. The cardioprotective mechanism maybe related to lipid peroxidation and the reduction of apoptosis rate.