| 水稻染色体DNA的交变脉冲电泳分析 |
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| 引用本文： | 钟翎,李文哲,刘良式.水稻染色体DNA的交变脉冲电泳分析[J].生物化学与生物物理进展,1992,19(5):352-356. |
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| 作者姓名： | 钟翎 李文哲 刘良式 |
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| 作者单位： | 中山大学生物工程研究中心;中山大学生物工程研究中心;中山大学生物工程研究中心 |
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| 基金项目： | 国家高科技“863计划”资助项目部分内容 |
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| 摘 要： | 从水稻完整的细胞核中释放出来的染色体DNA在交变脉冲电泳申表现为一种“240kb单位”的形式。这种单位,经限制性内切酶消化,可产生连续分布的大至1500kb的染色体DNA片段。文章讨论了“240kb单位”作为水稻染色体DNA基本结构单位的可能性。
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| 关 键 词： | 交变脉冲电泳 水稻 染色体DNA |
| 收稿时间： | 1991/6/25 0:00:00 |
| 修稿时间： | 2/9/1992 12:00:00 AM |
ANALYSIS OF CHROMOSOME DNA OF RICE USING PFGE TECHNIQUE |
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| Zhong Ling,Li Wenzhe and Liu Liangshi.ANALYSIS OF CHROMOSOME DNA OF RICE USING PFGE TECHNIQUE[J].Progress In Biochemistry and Biophysics,1992,19(5):352-356. |
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| Authors: | Zhong Ling Li Wenzhe and Liu Liangshi |
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| Institution: | Zhongshan University, Biotechnology Research Center;Zhongshan University, Biotechnology Research Center;Zhongshan University, Biotechnology Research Center |
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| Abstract: | Plant chromosome DNAs released from intact nuclei were analysed in PFGE and it showed that rice chromosome DNAs existed in a form of "240kb-like units" in a releasing condition of this experiments. These units with restriction enzyme digestion were shown to be chromosome-size DNAs up to 1500kb. The possibility of these "240kb-like units" as fundamental organization units of chromosome DNAs was discussed. This technique has been established for a basis of isolating and purifying large DNA fragments, constructing YAC library and restriction enzyme analyzing. |
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| Keywords: | PFGE(pulse field gradient gel electrophoresis) rice chromosome DNA |
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