Knock-out of metacaspase and/or cytochrome c results in the activation of a ROS-independent acetic acid-induced programmed cell death pathway in yeast |
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Authors: | Nicoletta Guaragnella Ersilia Marra |
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Institution: | a CNR, Istituto di Biomembrane e Bioenergetica, Via Amendola 165/A, 70126 Bari, Italy b Dipartimento di Scienze per la Salute, Università del Molise, I-86100 Campobasso, Italy |
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Abstract: | To gain further insight into yeast acetic acid-induced programmed cell death (AA-PCD) we analyzed the effects of the antioxidant N-acetyl-l-cysteine (NAC) on cell viability, hydrogen peroxide (H2O2) production, DNA fragmentation, cytochrome c (cyt c) release and caspase-like activation in wild type (wt) and metacaspase and/or cyt c-lacking cells. We found that NAC prevents AA-PCD in wt cells, by scavenging H2O2 and by inhibiting both cyt c release and caspase-like activation. This shows the occurrence of a reactive oxygen species (ROS)-dependent AA-PCD. Contrarily no NAC dependent change in AA-PCD of mutant cells was detectable, showing that a ROS-independent AA-PCD can also occur. |
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Keywords: | Yeast Programmed cell death Reactive oxygen species l-cysteine" target="_blank">N-Acetyl-l-cysteine Cytochrome c YCA1 |
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