Loss of Trx-2 enhances oxidative stress-dependent phenotypes in Drosophila |
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Authors: | Manabu Tsuda Chiaki Ohkura Ki-Hyeon Seong Toshiro Aigaki |
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Institution: | a Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji-shi, Tokyo 192-0397, Japan b Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305-0074, Japan |
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Abstract: | Overexpression of thioredoxin (TRX) confers oxidative stress resistance and extends lifespan in mammals and insects. However, less is known about phenotypes associated with loss of TRX. We investigated loss-of-function phenotypes of Trx-2 in Drosophila, and found that the mutant flies are hyper-susceptible to paraquat, a free radical generator, but not to hydrogen peroxide. They contain a high amount of protein carbonyl, which dramatically increases with age. Trx-2 mutants express high levels of anti-oxidant genes, such as superoxide dismutase, catalase, and glutathione synthetase. This is the first demonstration of biochemical and physiological consequences caused by loss of Trx-2 in Drosophila. |
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Keywords: | TRX Thioredoxin ROS reactive oxygen species Sod superoxide dismutase cat catalase GS glutathione synthetase RT-PCR real-time polymerase chain reaction UAS upstream activating sequence |
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