A novel splice variant of the human dicer gene is expressed in neuroblastoma cells |
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Authors: | Nicoletta Potenza Paola Scaruffi Gian Paolo Tonini |
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Affiliation: | a Department of Life Sciences, Second University of Naples, Via Vivaldi 43, 81100 Caserta, Italy b Translational Paediatric Oncology, National Cancer Research Institute (IST), Largo R. Benzi 10, Genoa 16132, Italy |
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Abstract: | Dicer is a ribonuclease playing a key role in the biogenesis of microRNAs and small interfering RNAs. Here we report the identification of a novel splice variant of human dicer gene, the first one bearing a modified coding sequence. It encodes a truncated protein, t-Dicer that lacks the dsRNA-binding domain and is defective in one of the two RNase III catalytic centers. The splice variant was found in neuroblastoma cells and in cells induced to neuronal differentiation, whereas it was not detectable in other cell lines or in normal tissues. Interestingly, it occurred in primary neuroblastic tumors, mainly in stroma poor neuroblastomas. |
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Keywords: | Dicer miRNA Neuroblastoma Neural differentiation RNase Alternative splicing |
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