Identification of a novel splicing isoform of murine CGI-58 |
| |
Authors: | Xingyuan Yang |
| |
Institution: | Department of Pediatrics, University of Kentucky, Lexington, KY, USA Kentucky Pediatric Research Institute, University of Kentucky, Lexington, KY, USA |
| |
Abstract: | The comparative gene identification-58 (CGI-58) gene, mutations of which are linked to Chanarin-Dorfman syndrome, encodes a protein of the α/β hydrolase domain subfamily. We report here a new alternative splicing isoform of the murine CGI-58 gene, termed mCGI-58S. Sequence comparison indicates the lack of second and third exons in this cDNA variant. While the full-length protein displayed perilipin-dependent localization to lipid droplets, mCGI-58S showed a predominant cytoplasmic staining when expressed in cells. mCGI-58S was incapable of activating adipose triglyceride lipase but retained the capacity to acylate lysophosphatidic acid. Overexpression of mCGI-58S failed to promote lipid droplet turnover and loss of intracellular triacylglycerols. These results suggest that this splicing event may be involved in the regulation of lipid homeostasis. |
| |
Keywords: | CGI-58 comparative gene identification-58 CDS Chanarin-Dorfman syndrome LPA lysophosphatidic acid PA phosphatidic acid ATGL adipose triglyceride lipase TAG triacylglycerol |
本文献已被 ScienceDirect 等数据库收录! |
|