Heterologous expression system in <Emphasis Type="Italic">Aspergillus oryzae</Emphasis> for fungal biosynthetic gene clusters of secondary metabolites |
| |
Authors: | Kanae Sakai Hiroshi Kinoshita Takuya Nihira |
| |
Institution: | (1) International Center for Biotechnology, Osaka University, 2-1 Yamadaoka Suita, Osaka 565-0871, Japan;(2) MU-OU Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd, 10400 Bangkok, Thailand; |
| |
Abstract: | Fungal secondary metabolites have been considered promising resources in the search for novel bioactive compounds. Given the
high potential of fungi as genetic resources, it is essential to find an efficient way to link biosynthetic genes to the product
in a heterologous system, because many genes for the secondary metabolite in the original strain are silent under standard
laboratory conditions. In a previous study, we constructed a heterologous expression system for a biosynthetic gene cluster
using Aspergillus oryzae as the host. To make the host more versatile for the expression of secondary metabolism genes, the expression levels of a
global regulator, laeA, were increased by placing the A. oryzae laeA gene under the control of the constitutive active pgk promoter. In the A. oryzae overexpressing laeA, two clusters of heterologous biosynthetic genes the monacolin K (MK) gene cluster from Monascus pilosus and the terrequinone A (TQ) gene cluster from Aspergillus nidulans] were successfully overexpressed, resulting in the production of the corresponding metabolite, MK or TQ. The successful production
of secondary metabolites belonging to different structural groups, namely MK as a polyketide and TQ as a hybrid of amino acid
and isoprenoid, indicated that the laeA-enriched A. oryzae was a versatile host for the heterologous expression of the biosynthetic gene cluster. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|