Plant-based strategies aimed at expressing HIV antigens and neutralizing antibodies at high levels. Nef as a case study |
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Authors: | Carla Marusic Alessandro Vitale Emanuela Pedrazzini Marcello Donini Lorenzo Frigerio Ralph Bock Philip J Dix Matthew S McCabe Michele Bellucci Eugenio Benvenuto |
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Institution: | 1. Dipartimento BAS, Sezione Genetica e Genomica Vegetale, ENEA, C.R. Casaccia, via Anguillarese 301, 00123, Rome, Italy 2. Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133, Milan, Italy 3. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK 4. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany 5. Department of Biology, National University of Ireland Maynooth, Maynooth, Co., Kildare, Ireland 6. Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, via della Madonna Alta 130, 06128, Perugia, Italy
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Abstract: | The first evidence that plants represent a valid, safe and cost-effective alternative to traditional expression systems for
large-scale production of antigens and antibodies was described more than 10 years ago. Since then, considerable improvements
have been made to increase the yield of plant-produced proteins. These include the use of signal sequences to target proteins
to different cellular compartments, plastid transformation to achieve high transgene dosage, codon usage optimization to boost
gene expression, and protein fusions to improve recombinant protein stability and accumulation. Thus, several HIV/SIV antigens
and neutralizing anti-HIV antibodies have recently been successfully expressed in plants by stable nuclear or plastid transformation,
and by transient expression systems based on plant virus vectors or Agrobacterium-mediated infection. The current article gives an overview of plant expressed HIV antigens and antibodies and provides an
account of the use of different strategies aimed at increasing the expression of the accessory multifunctional HIV-1 Nef protein
in transgenic plants. |
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Keywords: | HIV Nef Multi-component vaccine Plant made pharmaceuticals Recombinant proteins Heterologous expression systems |
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