超氧物歧化酶(SOD)在大豆下胚轴线粒体内的定位

本文介绍一种简单有效的制备、分离植物线粒体分部的方法,根据这一方法证明了大豆下胚轴线粒体内的 SOD 主要在基质可溶性部分,是属于对氰化物不敏感的 Mn-SOD,它占线粒体 SOD 总活性的80%,其余 SOD 活性主要在线粒体的膜间空间,约占总活性的16%。结果表明,SOD 在植物线粒体内的分布和定位与动物组织相似。

THE INTRAMITOCHONDRIAL LOCALIZATION OF SUPEROXIDE DISMUTASE FROM HYPOCOTYL OF ETIOLATED SOYBEAN SEEDLINGS

In the KCN inhibition experiment,it was found that superoxide dismutase from the hypo- cotyl of etiolated soybean seedlings which is composed of 88% Mn-SOD and 12% Cu-Zn-SOD. Latent activity of SOD was evidently liberated after the mitochondria were treated by supersoni- cation.Most of SOD activity was present in supernatant fraction after the supersonicated mito- chondria were centrifuged at 150000g for 60min.The method of separation and praparation of ub-mitochondrial fraction is described in this paper,and the results indicate that the 80% of mitochondrial SOD present in mitochondrial matrix fraction and a small amount SOD(16%) present in the mitochondrial intermembrane space.The results indicate that distribution and localization of intramitochondrial SOD from higher plant tissues are similar to that from animal tissues.