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Detection and molecular cloning of CYP74Q1 gene: Identification of Ranunculus acris leaf divinyl ether synthase |
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| Authors: | Svetlana S Gorina Yana Y ToporkovaLucia S Mukhtarova Ivan R ChechetkinBulat I Khairutdinov Yuri V GogolevAlexander N Grechkin |
| Institution: | Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia |
| Abstract: | |
| Keywords: | DES divinyl ether synthase RaDES Ranunculus acris divinyl ether synthase HPL hydroperoxide lyase AOS allene oxide synthase 13(S)-HPOT (9Z 11E 13S 15Z)-13-hydroperoxyoctadecatrienoic acid 13(S)-HPOD (9Z 11E 13S)-13-hydroperoxyoctadecadienoic acid 9(S)-HPOD (9S 10E 12Z)-9-hydroperoxyoctadecadienoic acid IMAC immobilized metal affinity chromatography (IMAC) TMS trimethylsilyl GC-MS gas chromatography-mass spectrometry ECL equivalent chain lengths ORF open reading frame IHCD I-helix central domain the catalytically important six amino acid domain in the centre of P450 I-helix |
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