Detection of H-2K mRNA in mouse 8-cell embryo by cDNA cloning |
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| Authors: | T Nagata M Nozaki T Morita A Matsushiro |
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| Institution: | Department of Microbial Genetics, Osaka University. |
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| Abstract: | Mouse MHC class I gene expression in 8-cell embryo was examined by cDNA cloning. We constructed a cDNA library from 8-cell embryos of ICR mice and isolated a class I cDNA from 3.0 x 10(5) phage clones of the library. Sequencing analysis of this clone revealed it to include the cDNA fragment extending from the exon 6 of the cytoplasmic portion to 3' untranslated region 1 of H-2K gene. Qa, Tla or other embryonic class I cDNA have not been isolated in the library. |
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