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Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8'-hydroxylase CYP707A |
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| Authors: | Okazaki Mariko Nimitkeatkai Hataitip Muramatsu Taku Aoyama Hikaru Ueno Kotomi Mizutani Masaharu Hirai Nobuhiro Kondo Satoru Ohnishi Toshiyuki Todoroki Yasushi |
| Institution: | a Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan b Division of Bioresource Science, Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan c Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan d Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8501, Japan e Division of Global Research Leaders, Shizuoka University, Shizuoka 422-8529, Japan |
| Abstract: | We developed abscinazole-E1 (Abz-E1), a specific inhibitor of abscisic acid (ABA) 8′-hydroxylase (CYP707A). This inhibitor was designed and synthesized as an enlarged analogue of uniconazole (UNI), a well-known plant growth retardant, which inhibits a gibberellin biosynthetic enzyme (ent-kaurene oxidase, CYP701A) as well as CYP707A. Our results showed that Abz-E1 functions as a potent inhibitor of CYP707A and a poor inhibitor of CYP701A both in vitro and in vivo. Abz-E1 application to plants resulted in improved desiccation tolerance and an increase in endogenous ABA. |
| Keywords: | Plant growth regulator P450 inhibitor ABA |
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