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Biologically active constituents from the fruiting body of Taiwanofungus camphoratus |
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| Authors: | Shi Li-Shian Chao Chih-Hua Shen De-Yang Chan Hsiu-Hui Chen Chou-Hsiung Liao Yu-Ren Wu Shwu-Jen Leu Yann-Lii Shen Yuh-Chiang Kuo Yao-Haur Lee E-Jian Qian Keduo Wu Tian-Shung Lee Kuo-Hsiung |
| Affiliation: | a Department of Biotechnology, National Formosa University, Yunlin 632, Taiwan b Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan c Department of Medical Technology, Chung Hua University of Medical Technology, Tainan 717, Taiwan d Natural Products Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan 333, Taiwan e National Research Institute of Chinese Medicine, Taipei 112, Taiwan f Department of Surgery, National Cheng Kung University Medical Center and Medical School, Tainan 701, Taiwan g Chinese Medicine Research and Development Center and Department of Pharmacy, China Medical University and Hospital, Taichung, Taiwan h Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599-7568, USA |
| Abstract: | Five new benzenoids, benzocamphorins A-E (1-5), and 10 recently isolated triterpenoids, camphoratins A-J (16-25), together with 23 known compounds including seven benzenoids (6-12), three lignans (13-15), and 13 triterpenoids (26-38) were isolated from the fruiting body of Taiwanofungus camphoratus. Their structures were established by spectroscopic analysis. Selected compounds were examined for cytotoxic and anti-inflammatory activities. Compounds 9 and 21 showed moderate cytotoxicity against MCF-7 and Hep2 cell lines with ED50 values of 3.4 and 3.0 ??g/mL, respectively. Compounds 21, 25, 26, 29-31, 33, and 36 demonstrated potent anti-inflammatory activity by inhibiting lipopolysaccharide (LPS)-induced nitric oxide (NO) production with IC50 values of 2.5, 1.6, 3.6, 0.6, 4.1, 4.2, 2.5, and 1.5 ??M, respectively, which were better than those of the nonspecific nitric oxide synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME) (IC50: 25.8 ??M). These results may substantiate the use of T. camphoratus in traditional Chinese medicine (TCM) for the treatment of inflammation and cancer-related diseases. The newly discovered compounds deserve further development as anti-inflammatory candidates. |
| Keywords: | Taiwanofungus camphoratus Fruiting bodies Anti-inflammatory NOX ROS Cytotoxicity |
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