米蛾体内Wolbachia的wsp基因序列测定与系统发育分析

Wolbachia是广泛分布于节肢动物体内的一类共生菌, 它们参与多种调控寄主的生殖活动机制。通过对wsp基因的特异性扩增和测序，发现了Wolbachia在米蛾Corcyra cephalonica (Stainton)体内的感染。利用所测序列和其他已发表的序列建立系统树，结果表明米蛾体内Wolbachia属于B大组的Pip类群，与其寄生物茧蜂及赤眼蜂中的Wolbachia各株系遗传距离相差较远。据此推测米蛾体内感染的Wolbachia不是由寄生物（茧蜂、赤眼蜂）水平传播所致。

Sequencing and phylogenetic analysis of the wsp gene of Wolbachia in Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae)

Wolbachia is a common and widespread group of bacteria found in arthropods. These bacteria have evolved various mechanisms for manipulating reproduction of their hosts. The presence of Wolbachia in a laboratory population of Corcyra cephalonica (Stainton) was firstly identified based on amplification and sequencing part of the wsp gene. Aligned the obtained sequence with the published ones, the phylogenetic relationships among Wolbachia found in C. cephalonica and other insects were established. The Wolbachia found in C. cephalonica belong to the Pip group, far separated from the groups of Wolbachia found in braconid wasps and Trichogramma spp. It was so inferred that Wolbachia did not transmit horizontally between C. cephalonica and its parasitoids (Apanteles sp. and Trichogramma spp.).