Ultrastructural alterations of the myocardium induced by doxorubicin |
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Authors: | Tsutomu Iwasaki Tadashi Suzuki |
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Institution: | Second Department of Internal Medicine, Gunma University School of Medicine, Japan. |
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Abstract: | Morphologic changes in Doxorubicin (DXR)-induced cardiomyopathy are characterized by marked dilatation of the sarcoplasmic reticulum (SR). DXR was administered to New Zealand White rabbits for 5 or 8 weeks and the three-dimensional structure of the sarcotubular system in cardiac muscle cells from each rabbit was examined under a field-emission type scanning electron microscope (SEM) after removal of cytoplasmic matrices by the osmium-DMSO-osmium procedure. Five weeks after the initial injection of DXR, partial dilatation of the SR and damaged mitochondria with lysis of cristae were observed three-dimensionally. After 8 weeks, the three-dimensional structure of the SR showed extensive spherical ballooning which could be seen clearly in bold relief. Thus, we could directly visualize structural alterations of the sarcotubular system in DXR-induced cardiomyopathy using the SEM. |
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Keywords: | Doxorubicin Cardiomyopathy Sarcoplasmic reticulum Scanning electron microscopy Threedimensional structure |
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