Metagenomic‐based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome |
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Authors: | Jean‐Michel Hily Sandrine Demanèche Nils Poulicard Mélanie Tannières Samia Djennane Monique Beuve Emmanuelle Vigne Gérard Demangeat Véronique Komar Claude Gertz Aurélie Marmonier Caroline Hemmer Sophie Vigneron Armelle Marais Thierry Candresse Pascal Simonet Olivier Lemaire |
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Institution: | 1. INRA, SVQV UMR‐A 1131, Université de Strasbourg, Colmar, France;2. Laboratoire Ampère (CNRS UMR5005), Environmental Microbial Genomics, école Centrale de Lyon, Université de Lyon, Ecully, France;3. IRD, Cirad, Univ. Montpellier, IPME, Montpellier, France;4. UMR 1332 Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, Villenave d'Ornon Cedex, France |
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Abstract: | For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus‐resistant transgenic plants based on the pathogen‐derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer (HGT) of any part of the transgene to an existing gene pool, have been raised. Here, we report no evidence of any undesirable impacts of genetically modified (GM) grapevine rootstock on its biotic environment. Using state of the art metagenomics, we analysed two compartments in depth, the targeted Grapevine fanleaf virus (GFLV) populations and nontargeted root‐associated microbiota. Our results reveal no statistically significant differences in the genetic diversity of bacteria that can be linked to the GM trait. In addition, no novel virus or bacteria recombinants of biosafety concern can be associated with transgenic grapevine rootstocks cultivated in commercial vineyard soil under greenhouse conditions for over 6 years. |
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Keywords: | environmental microbiology metagenomics grapevine transgenic rootstock safety and regulatory affairs |
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