碱性成纤维细胞生长因子对乳鼠心肌细胞缺氧复氧损伤的影响

本实验在培养的乳鼠心肌细胞缺氧复氧损伤模型上观察碱性纤维细胞生长因子对Ａ／Ｒ损伤及蛋白激酶活性的影响，以探讨ｂＥＧＦ作为药物预处理心肌保护的可行性及其机理。结果表明，ｂＥＧＦ预处理呈浓度依赖地提高Ａ／Ｒ后心肌细胞存活率，减少细胞内ＡＴＰ消耗及胞浆乳酸脱氢酶漏出；ＰＫＣ抑制剂Ｈ７完全消除ｇＦＧＦ的上述保护作用；实验结果表明，ｂＦＧＦ可以直接激活心肌细胞ＰＫＣ，其激活时相的变化与缺氧预处理者相近，提示

EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON ANOXIA/REOXYGENATION INJURY OF RAT NEONATAL CARDIOMYOCYTES

The effects of basic fibroblast growth factor (bFGF) on anoxia/reoxygenation(A/R) injury and protein kinase C (PKC) activity were studied on a model of A/R injury of neonatal rat cardiomyocytes to investigate the possibility of its using as a substrate for pharmacological preconditioning. The data indicated that bFGF improved theviability of cardiomyocytes, lowered the deplection of ATP and leakage of intracellularlactate dehydrogenase (LDH) in a concentration-dependent manner. PKC inhibitor, H7,completely abolished the protective effects. It was also found that bFGF directely activated PKC in cardiomyocytes in a time course similar to that in hypoxic preconditioning.The data suggested that the protective effect of bFGF on cardiomyocyte A/R injurymight be mediated by PKC.