Fixed bed porous glass sphere (porosphere) bioreactors for animal cells |
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Authors: | D Looby J B Griffiths |
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Institution: | (1) Biologics Division, PHLS CAMR, Porton Down, SP4 0JG Salisbury, Wiltshire, United Kingdom |
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Abstract: | Process intensity of fixed bed glass sphere culture systems is increased considerably by replacing solid glass spheres with open pore glass spheres. This technique demonstrates the possibility of having a system capable of both volumetric and cell density scale up and being suitable for substrate attached and suspension cells. The yields achieved for a number of attached cell lines (approximately 107/ml) demonstrate an increase approaching one order of magnitude over solid glass spheres (approximately 106/ml). Also suspension cells were successfully entrapped in the open pore structure with similar yields. |
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Keywords: | fixed bed immobilisation perfusion scale-up of cell cultures |
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