A chondroitin sulphate proteoglycan from human cultured glial cells aggregates with hyaluronic acid |
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| Authors: | Börje Norling Bengt Glimelius Bengt Westermark Åke Wasteson |
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| Affiliation: | 1. Institute of Medical and Physiological Chemistry, University of Uppsala, Uppsala, Sweden;2. the Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden |
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| Abstract: | Media harvested from cultures of glial cells grown in the presence of 35S-sulphate were shown to contain 35S-labelled proteoglycans. One of the components was a chondroitin sulphate proteoglycan that had an apparent monomer size similar to that of cartilage-derived chondroitin sulphate proteoglycan. The glial proteoglycan formed aggregates in the presence of hyaluronic acid; aggregation was abolished in the presence of deca- to tetradecasaccharides derived from hyaluronic acid or by previous reduction and alkylation of the proteoglycan. It is concluded that the ability to produce large chondroitin sulphate proteoglycan molecules capable of binding to hyaluronic acid is not confined to cartilage cells. |
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