首页 | 本学科首页   官方微博 | 高级检索  
     


Lobeline production by hairy root culture of Lobelia inflata L.
Authors:Hiroshi Yonemitsu  Koichiro Shimomura  Motoyoshi Satake  Shunji Mochida  Masahiko Tanaka  Thoru Endo  Akira Kaji
Affiliation:(1) Research Institute for Molecular Genetics, Tsumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki, 300-11 Ibaraki, Japan;(2) Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305 Ibaraki, Japan;(3) Department of Microbiology, School of Medicine, University of Pennsylvania, 19104-6076 Philadelphia, PA, USA
Abstract:Hairy roots were obtained following inoculation of the stems of Lobelia inflata L. with Agrobacterium rhizogenes strain ATCC 15834. These hairy roots contained agropine and mannopine. In addition, lobeline was detected by HPLC and confirmed by mass spectrometry. Various media were tested for the growth of hairy roots as well as for the content of lobeline in hairy roots. The growth rate of hairy roots cultured in Nitsch and Nitsch's medium was approximately one third of those cultured in other media. The lobeline content of hairy roots (18–54 mgrg/g dry weight) cultured in these media was the same order of magnitude compared with that of roots of L. inflata (24 mgrg/g dry weight) cultivated in pots. The hairy roots cultured in Nitsch and Nitsch's medium were morphologically different from those cultured in other media.Abbreviations MS medium Murashige and Skoog's medium - 1/2 MS medium one-half strength of the standard Murashige and Skoog's medium - B5 medium Gamborg's B5 medium - NN medium Nitsch and Nitsch's medium - FW fresh weight - DW dry weight
Keywords:Lobelia inflata L.  hairy root  lobeline  root morphology
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号