Morphology of a human-derived YAC in yeast meiosis |
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Authors: | Josef Loidl Harry Scherthan Johan T Den Dunnen Franz Klein |
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Institution: | (1) Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria;(2) Division of Human Biology and Human Genetics, The University, Erwin Schroedinger-Strasse, D-67663 Kaiserslautern, Germany;(3) Department of Human Genetics, Leiden University, Wassenaarseweg 27, NL-2333 AL Leiden, The Netherlands |
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Abstract: | In meiosis of human males DNA is packaged along pachytene chromosomes about 20 time more compactly than in meiosis of yeast. Nevertheless, a human-derived yeast artificial chromosome (YAC) shows the same degree of compaction of DNA as endogenous chromosomes in meiotic prophase nuclei of yeast. This suggests that in yeast meiosis, human and yeast DNA adopt a similar organization of chromatin along the pachytene chromosome cores. Therefore meiotic chromatin organization does not seem to be an inherent chromosomal property but is governed by the host-specific cellular environment. We suggest that there is a correlation between the less dense DNA packaging and the increased rate of recombination that has been reported for human-derived YACs as compared with human DNA in its natural environment. |
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