Random integration of SV40 in SV40-transformed, immortalized human fibroblasts |
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| Authors: | Hiroto Hara Hideko Kaji |
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| Affiliation: | 1. Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 USA;2. Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742 USA;3. Materials Science and Engineering Department, University of Maryland, College Park, MD 20742 USA;4. Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 USA |
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| Abstract: | We have studied the relationship between immortalization of SV40-transformed human embryonic fibroblasts and their SV40 integration sites. From several independently transformed cell pools, we have isolated clones which do not harbor unintegrated SV40 DNA. We have analysed whole-cell DNA from these clones, using the Southern blot method. Our results suggest that no specific integration sites in the cellular genome exist which are a prerequisite for the immortalization process. Although some integration sites were found to be predominant in pre-crisis clones, they could not be detected in the post-crisis clones. This suggests that none of these predominating sites is selected for during the crisis period. |
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