A molecular,isozyme and morphological map of the barley (Hordeum vulgare) genome |
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| Authors: | A Kleinhofs A Kilian M A Saghai Maroof R M Biyashev P Hayes F Q Chen N Lapitan A Fenwick T K Blake V Kanazin E Ananiev L Dahleen D Kudrna J Bollinger S J Knapp B Liu M Sorrells M Heun J D Franckowiak D Hoffman R Skadsen B J Steffenson |
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| Institution: | (1) Department of Crop and Soil Sciences, Washington State University, 99164-6420 Pullman, WA, USA;(2) Department of Genetics and Cell Biology, Washington State University, 99164-6420 Pullman, WA, USA;(3) Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, 24061 Blacksburg, VA, USA;(4) Crop Sciences Department, Oregon State University, 97331 Corvalis, OR, USA;(5) Department of Agronomy, Colorado State University, 80523 Fort Collins, CO, USA;(6) Department of Plant and Soil Sciences, Montana State University, 59715 Bozeman, MT, USA;(7) USDA-ARS, Northern Crop Science Laboratory, 58105 Fargo, ND, USA;(8) Department of Plant Breeding, Cornell University, 14853-1902 Ithaca, NY, USA;(9) Department of Crop and Weed Sciences, North Dakota State University, 58105-5051 Fargo, ND, USA;(10) USDA-ARS, National Small Grains Germplasm Research Facility, 83210 Aberdeen, ID, USA;(11) Cereal Crops Research Unit, 53705-2334 Madison, WI, USA;(12) Department of Plant Pathology, North Dakota State University, 58105 Fargo, ND, USA;(13) Present address: Department of Genetics, Silesian University, Katowice, Poland;(14) Present address: Department of Plant Pathology, North Carolina State University, 27695-7616 Raleigh, NC, USA |
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| Abstract: | A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.This research was also supported by other members of the NABGMP: K. Kasha, Department of Crop Science, University of Guelph, Guelph, Ontario, Canada NIG 2W1; W. Kim, Agriculture Canada Research Station, 195 Dafoe Road, Winnipeg, Manitoba, Canada R3T 2M9; A. Laroche, Agriculture Canada Research Station, P.O. Box 3000 Main, Lethbridge, Alberta, Canada,TU 4B1; S. Molnar, Plant Research Centre Agriculture Canada, Central Experimental farm, Ottawa, Ontario, Canada K1A 0C6; G. Scoles, Department of Crop Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N OWOThis research is part of the North American Barley Genome Mapping Project, R. A. Nilan and K. Kasha, Coordinator and Associate Coordinator, respectively
Permanent address: Department of Plant Genetics, NI Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow |
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| Keywords: | RFLP Mapping Barley Genome Centromeres |
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