Demonstration of hydrogenase electrode operation in a bioreactor |
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| Authors: | Shastik E S Vokhmyanina D V Zorin N A Voronin O G Karyakin A A Tsygankov A A |
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| Institution: | a Institute of Basic Biological Problems, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia
b Chemical Faculty, Moscow State University, 119992 Moscow, Russia |
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| Abstract: | This work describes the first step towards combination of the bioreactor with a starch-degrading microbial consortium and hydrogenase electrode (HE) in one unit for electricity generation. For this purpose, the bioreactor for microbial fermentation was designed with a set of electrodes (pH-sensor, Ag|AgCl reference electrode, Pt-electrode, and HE) inside the bioreactor. Potentials of all electrodes and H2 accumulation were monitored in the system under the precise pH control. Results obtained with the hydrogen-producing microbial consortium indicated that HE generates the potential equal to the H2|2H+ equilibrium potential. Furthermore, HE was able to catalyze the current generation (200 μA) by consuming H2 gas produced in the microbial consortium from starch. After 220 h of operation, HE retained at least 81% of the initial activity. Calculations of carbon balance indicated that fermentation products were similar in microbial cells without HE and with HE generating the current due to H2 consumption. |
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| Keywords: | Dark fermentative hydrogen production Hydrogenase electrode Electricity generation from biohydrogen |
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