Optimization of ultrasonic circulating extraction of polysaccharides from Asparagus officinalis using response surface methodology |
| |
Authors: | Zhao Qingsheng Kennedy John F Wang Xiaodong Yuan Xiaofan Zhao Bing Peng Youshun Huang Yunxiang |
| |
Institution: | a National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, PR China b Graduate University of Chinese Academy of Sciences, 100049 Beijing, PR China c Chembiotech Laboratories, Institute of Advanced Science and Technology, The Croft, Buntsford Drive, Stoke Heath, Bromsgrove, Worcestershire B60 4JE, UK d Hebei Normal University of Science & Technology, Qinhuangdao 066006, PR China e Qinhuangdao Chang Sheng Agricultural Science and Technology Co., Ltd., Qinhuangdao 066006, PR China |
| |
Abstract: | Polysaccharides were extracted from Asparagus officinalis. A novel ultrasonic circulating extraction (UCE) technology was applied for the polysaccharide extraction. Three-factor-three-level Box-Behnken design was employed to optimize ultrasonic power, extraction time and the liquid-solid ratio to obtain a high polysaccharide yield. The optimal extraction conditions were as follows: ultrasonic power was 600 W, extraction time was 46 min, the liquid-solid ratio was 35 mL/g. Under these conditions, the experimental yield of polysaccharides was 3.134%, which was agreed closely to the predicted value. The average molecular weight of A. officinalis polysaccharide was about 6.18 × 104 Da. The polysaccharides were composed of glucose, fucose, arabinose, galactose and rhamnose in a ratio of 2.18:1.86:1.50:0.98:1.53. Compared with hot water extraction (HWE), UCE showed time-saving, higher yield and no influence on the structure of asparagus polysaccharides. The results indicated that ultrasonic circulating extraction technology could be an effective and advisable technique for the large scale production of plant polysaccharides. |
| |
Keywords: | Ultrasonic circulating extraction Asparagus officinalis Polysaccharides Response surface methodology |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|