Selection of protoclones for high yields of indole alkaloids from suspension cultures ofCatharanthus roseus and the qualitative analysis of the compounds by LC-MS |
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| Authors: | K H Jung S S Kwak S W Kim C Y Choi G S Heo J R Liu |
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| Institution: | (1) Plant Cell Biology Laboratory, Genetic Engineering Research Institute, Korea Institute of Science and Technology, Taedok Science Town, P.O. Box 17, 305-606 Taejon, Korea;(2) Biochemical Engineering Major, Seoul National University, 151-742 Seoul, Korea;(3) Korea Standard Research Institute, Taedok Science Town, P.O. Box 3, 305-606 Taejon, Korea |
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| Abstract: | Summary To produce economically important indole alkaloids by cell culture, we have selected protoclones ofCatharanthus roseus for high yields of catharanthine and ajmalicine. Protoplasts were enzymatically isolated from suspension-cultured cells.
Protoclone VPC-10 produced catharanthine at 5.9 μg/g fresh wt of cells after 10 days of culture, although the original cell
line did not produce it at a level detectable by HPLC. Under the same conditions, protoclone VPC-15 produced ajmalicine at
133.6 μg/g, which was about 3 times the productivity of the original cell line. In addition, the indole alkaloids were qualitatively
confirmed by LC-MS. |
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