Identification of gibberellin A4 in Pisum sativum L. and the effects of applied gibberellins A9, A4, A5 and A3 on the le mutant |
| |
Authors: | Andrew T Poole John J Ross Naomi L Lawrence James B Reid |
| |
Institution: | (1) Department of Plant Science, University of Tasmania, G.P.O. Box 252C, 7001 Hobart, Tasmania, Australia |
| |
Abstract: | Gibberellin A4 (GA4) was identified for the first time in the garden pea (Pisum sativum) L.), by gas chromatography-mass spectrometry. However, in wild-type shoots the level of GA4 was only about 6% of the level of GA1, and it is therefore unlikely that GA4 plays a major role per se in the control of pea stem elongation. In shoots of the le mutant, GA4 was not detected, while the level of GA9 was approximately twice that found in the wild-type. The le mutation also markedly reduced the elongation response to applied GA9. It appears, therefore, that in Pisum the le mutation blocks the 3-hydroxylation of GA9 to GA4, in addition to the 3-hydroxylation of GA20 to GA1. In contrast, the le mutation did not reduce the response to applied GA5, suggesting the step GA5 to GA3 is not catalysed by the enzyme controlled by the Le gene. The step GA5 to GA3 was confirmed in peas by metabolite analysis after treatment with deuterated GA5. |
| |
Keywords: | elongation gibberellins A4 A9 A5 and A3 le mutant Pisum |
本文献已被 SpringerLink 等数据库收录! |
|