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Twelve new polymorphic microsatellite markers from the loggerhead sea turtle (Caretta caretta) and cross-species amplification on other marine turtle species |
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| Authors: | Catalina Monzón-Argüello Joaquín Muñoz Adolfo Marco Luis Felipe López-Jurado Ciro Rico |
| Affiliation: | 1. Instituto Canario de Ciencias Marinas, Crta. De Taliarte s/n, 35200, Telde, Gran Canaria, Spain 2. Estación Biológica de Do?ana (CSIC), Avda. María Luisa s/n, 41013, Sevilla, Spain 3. Dpto de Biología Animal, Universidad de Las Palmas de G.C., Campus de Tafira, 35017, Las Palmas de Gran Canaria, Gran Canaria, Spain |
| Abstract: | We describe 12 new polymorphic dinucleotide microsatellite loci and multiplex Polymerase Chain Reaction conditions from the loggerhead sea turtle Caretta caretta. Levels of polymorphism were assessed in 50 individuals from the nesting population of the Cape Verde Islands. Number of alleles ranged from 3 to 13 (average of 7.33) and the values of observed heterozygosities from 0.32 to 0.80 (average of 0.61). Cross-species amplification on three other marine turtles, Chelonia mydas, Eretmochelys imbricata and Dermochelys coriacea, revealed polymorphism and variability at eight, eleven and three loci, respectively. |
| Keywords: | Microsatellites Caretta caretta Marine turtle Dinucleotide repeats |
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