Phylogenetics of an antibiotic producing Streptomyces strain isolated from soil

Traditional methods of species classification and identification of the organism are based on morphological, physiological, biochemical,developmental and nutritional characteristics. Accurate assignment of taxonomic status to the new biologically active microbial isolates throughexisting bioinformatics methods is now very essential and also helpful in chemical characterization of the active molecule produced bymicroorganisms. The bacterial strain M4 (ckm7) was isolated from the pre-treated soil sample collected from the agricultural field of EasternUttar Pradesh (U.P.), India and was found to be producing antibacterial and antifungal antibiotics. Taxonomic identification of the isolate belongsto the genus Streptomyces which was done with the help of sequence analysis and later confirmed by biological activity. Sequence comparisonstudy of ckm7 showed 98% identical similarity with 16S rRNA gene sequences of Streptomyces spinichromogenes, Streptomyces triostinicus andStreptomyces capoamus. On the basis of both biological activity and phylogenetic analysis of ckm7, it was concluded that the isolated strain is anew variant of S. triostinicus.