Real-time DNA microarray analysis |
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| Authors: | Arjang Hassibi Haris Vikalo José Luis Riechmann Babak Hassibi |
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| Affiliation: | 1.Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, 2.Electrical Engineering Department, California Institute of Technology, Pasadena, CA 91125, 3.Electrical and Computer Engineering Department, University of Texas at Austin, Austin, TX 78712 and 4.Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA |
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| Abstract: | We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e. real-time DNA microarrays, enhances the detection dynamic range of conventional systems by being impervious to probe saturation in the capturing spots, washing artifacts, microarray spot-to-spot variations, and other signal amplitude-affecting non-idealities. We demonstrate in both theory and practice that the time-constant of target capturing in microarrays, similar to all affinity-based biosensors, is inversely proportional to the concentration of the target analyte, which we subsequently use as the fundamental parameter to estimate the concentration of the analytes. Furthermore, to empirically validate the capabilities of this method in practical applications, we present a FRET-based assay which enables the real-time detection in gene expression DNA microarrays. |
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