Light-Dependent Transformation of Aniline to Indole Esters by the Purple Bacterium Rhodobacter sphaeroides OU5 |
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Authors: | Vijay Shanker Sunayana Mandala Rayabandla Ranjith Nayak Kumavath Sasikala Chintalapati Ramana Chintalapati |
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Institution: | (1) Department of Plant Sciences, School of Life Sciences, University of Hyderabad P.O. Central University, Hyderabad, 500 046, India;(2) Environmental Microbial Biotechnology Laboratory, Center for Environment, IST, J NT University, Kukatpally, Hyderabad, 500 072, India |
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Abstract: | In an attempt to understand the aromatic hydrocarbon metabolism by purple bacteria that do not grow at their expense, we earlier
reported 2-aminobenzoate transformation by a purple non-sulfur bacterium, Rhodobacter sphaeroides OU5 (Sunayana et al., 2005, J Ind Microbiol Biotech 32:41–45), which is extended in the present study with aniline, a major
environmental pollutant. Aniline did not support photo (light anaerobic) or chemo (dark aerobic) heterotrophic growth of Rhodobacter sphaeroides OU5 either as a sole source of carbon or nitrogen. However, light-dependent aniline transformation was observed in the culture
supernatants and the products were identified as indole derivatives. The transformation was dependent on a tricarboxylate
intermediate, fumarate. Five intermediates of the aniline biotransformation pathway were isolated and identified as indole
esters having a mass of 443, 441, 279, 189, and 167 with unstoichiometric total indole yields of 0.16 mM from 5 mM of aniline consumed. The pathway proposed based on these intermediates suggest a novel xenobiotic detoxification process
in bacteria. |
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